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辣根过氧化物酶标记的小鼠抗His6标签单克隆抗体
所属类别：特色试剂
描述：抗His6标签单克隆抗体（HRP标记）
详细介绍
| 产品信息 | |
| 中文名称 | 辣根过氧化物酶标记的小鼠抗His6标签单克隆抗体 |
| 英文名称 | HRP-conjugated mouse Anti-His6 tag Monoclonal Antibody |
| 货号 | Abt-P-3534 |
| 种属来源 | Mouse |
| 抗体亚型 | IgG2b/kappa |
| 克隆号 | H6 |
| 免疫原 | 6×His合成多肽(HHHHHH) |
| 分子量 | 150Kd |
| 纯化方式 | Protein G |
| 纯度 | >95%（SDS-PAGE） |
| 产品形式 | Liquid |
| 规格 | 100 ul/500 ul/1 ml |
| 保存缓冲液 | PBS with 0.02%sodium azide and 50%glycerol, pH7.3 |
| 保存 | Store at -20°C. |
| 应用 | WB/ELISA/Fc/IP |
产品说明
WB：
Conclusion：Our HRP-conjugated mouse Anti-His6 tag Monoclonal Antibody can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Direct ELISA：
基本信息
Protein tags are protein or peptide sequenceslocated either on the C- or N- terminal ofthe target protein, which facilitates one orseveral ofthe following characteristics:solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues bindsto several types ofimmobilized metal ions, including nickel, cobalt and copper, underspecific buffer conditions. The His-tag antibody is a useful tool for monitoring ofthe His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions offusion proteins expressed in bacteria, insect, and mammalian cells.